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- [ Indy Air Thermocycler ]
- [ RapidCycler ]

RapidCycler

  • Engineered to match the speed of biochemistry
  • Combining the ideal sample container with the ultimate heating and cooling medium — high velocity air
  • The standard for more than 5 years
  • 10 per/sec Peak Rate
  • High Temperature DNA Amplification
  • 30 Cycles in 8 minutes
  • 48 Sample Capacity
  • User Friendly Interface
  • Maximum Run 1,536 Samples per Day
  • 99 Cycle Programs

"The RapidCycler can complete a 30 cycle reaction in less than 10 minutes. Finally, a machine that can keep up with the speed of the biochemistry."

Efficiency
Heat block cyclers can take hours to do 30, three temperature cycles. This slow rate leads to long lines for the machine and sign up sheets that force you to start your reaction at 2 a.m.. The RapidCycler™ can complete a 30 cycle reaction in less than 10 minutes. Finally, a machine that can keep up with the speed of the biochemistry.

Why are other thermocyclers so slow? Thermocyclers generally use microcentrifuge tubes or modified microtiter plates. These plastic containers insulate the sample. The temperature of a 100 l sample within a microcentrifuge tube lags 20-40 secs behind the block temperature. This, combined with sluggish temperature ramp rates of heating blocks, explains why they are so slow.

There is another major factor - temperature hold times. A common protocol for temperature cycling is 1 min at 94C, 2 min at 55C and 3 min at 72C. Even if instantaneous temperature transitions were possible, one cycle would take 6 minutes. The actual enzymatic extension occurs in about 10 seconds for a 500 bp fragment. This leaves 97% of the total time wasted, not even counting ramp time. What are these machines doing? They are waiting for steady-state conditions to bring all the samples to roughly the same temperature. Unfortunately, because of the geometry of the heating elements and metal blocks, even the steady-state condition may not produce uniform temperatures across all samples. At best, these machines suffer from temperature imbalance during the long wait for equilibrium conditions. At worst, the imbalance is permanent.

Savings
The high sample throughput of the RapidCycler allows more users per machine.

  • Optimized for 10l reaction volumes saving you on reagent costs.
  • 25 and 50l capillaries and thin walled microcentrifuge tubes also provide excellent results.

Maximum Yield
As a reaction proceeds, the efficiency decreases. This is due in part to competition between primers and reaction product. Rapid cool-down (>5C/sec) favors the kinetics of primer annealing over the thermodynamic advantage of product reannealing. This in turn yields a higher final concentration of product.

Cleaner Product
Rapid cycling leaves less time for primer extension at non-specific annealing sites. The amount of nonspecific product is directly related to the time spent at low temperatures.

  • Cleaner electrophoresis gels
  • More readable gels
  • Programmable hold times
  • Maximum specificity

 

SPECIFICATIONS
Cycle Time Between 20-60 sec. for most applications. Thirty 3-temp. cycles in 10-30 min.
Min. Cycle Times Less than 15 sec. (94 for 0 sec., 65 for 0 sec.) Thirty cycles in < 8 minutes.
Temperature Ramping 10C/sec. peak rate. 99 selectable rates.
Programming Single screen programming. All cycle parameters are displayed on a single 4 x 20 LCD screen. Sixteen button keypad with cursor keys for convenient user input.
Program capacity 99 cycle programs, 99 hold programs, 99 link programs

Max. Cycles

99 per cycles program

Max. Hold Times

99 hours, 59 min 59 sec

Linking

Up to 10 cycle or hold programs can be linked together. Capable of storing up to 99 linked programs.
Max. Throughput 1,536 samples per 8 hour day (15 min. runs of 48 samples each)
Sample Capacity 48 samples
Sample Handling Glass microcapillary tubes (5-75l) or thin walled microcentrifuge tubes (10-200l). The capillary tubes are held at microtiter spacing for simultaneous sample pickup, cycling, and delivery. An optional buffer system allows direct loading of sample onto gels without further manipulation.
Temperature Profile  

Accuracy

0.5C

Resolution

1C

Variation

Between and within sample < 1C
Time Resolution 0.1 sec.
Dimensions Height: 12", Width: 9", Depth: 15" (30 x 23 x 37 cm), 26.5 lbs. (12 kg)
Power Requirements 100-120 VAC, 8.0A or 220-230 VAC, 4.0A
Warranty 1 year, parts and labor